Simplifying Genomic DNA Purification: Introducing the Maxi Whole Blood Genomic DNA Purification Kit AffiPURE

The Maxi Whole Blood Genomic DNA Purification Kit AffiPURE is designed to provide a rapid and efficient method for the isolation of high-quality genomic DNA from large volumes of whole blood. This article details the technical specifications, methodology, and performance metrics of the kit, making it accessible for laboratory personnel and researcher. Genomic DNA purification is a critical step in many molecular biology and genetics applications. The Maxi Whole Blood Genomic DNA Purification Kit AffiPURE addresses the need for high-quality DNA extraction from whole blood samples, which is essential for downstream applications such as PCR, qPCR, and next-generation sequencing.

Key Features

  • Spin Column-Based Purification: The kit utilizes spin columns packed with a proprietary affinity matrix that selectively binds genomic DNA, allowing for efficient separation from other cellular components.
  • User-Friendly Protocol: The purification protocol is straightforward and can be completed in a few simple steps, reducing the likelihood of user errors and ensuring consistent results.
  • High Yield and Purity: The AffiPURE technology ensures high yields of pure genomic DNA, suitable for downstream applications such as PCR, sequencing, and genotyping.
  • Compatibility: The kit is compatible with various blood sample types, including fresh whole blood, frozen blood, and blood stored in anticoagulant solutions, offering versatility to researchers working with different sample sources.

Protocol Overview

  • Blood Lysis: The whole blood sample is lysed using a lysis buffer supplied in the kit, releasing cellular contents including genomic DNA.
  • Binding: The lysate is then mixed with a binding buffer and loaded onto the spin column, where genomic DNA selectively binds to the affinity matrix.
  • Washing: Unbound impurities are removed through a series of wash steps, ensuring the purity of the isolated genomic DNA.
  • Elution: Pure genomic DNA is eluted from the spin column using a low-salt elution buffer, ready for downstream applications.


The purified genomic DNA obtained using the Maxi Whole Blood Genomic DNA Purification Kit AffiPURE is suitable for a wide range of molecular biology applications, including:

  • Polymerase Chain Reaction (PCR)
  • Restriction Fragment Length Polymorphism (RFLP) analysis
  • DNA sequencing
  • Genotyping
  • Southern blotting

Materials and Methods

Kit Components

  • Lysis Buffer
  • Binding Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Elution Buffer
  • Proteinase K
  • Spin Columns
  • Collection Tubes

Sample Preparation

  1. Starting Material: Whole blood samples (1-10 mL).
  2. Initial Lysis: Add Lysis Buffer and Proteinase K to the blood sample. Incubate at 56°C for 30 minutes to ensure complete lysis of blood cells.

DNA Binding

  1. Binding: Add Binding Buffer to the lysate. Mix thoroughly.
  2. Column Loading: Transfer the mixture to a spin column placed in a collection tube. Centrifuge at 6000 x g for 1 minute to bind DNA to the column membrane.


  1. First Wash: Add Wash Buffer 1 to the column. Centrifuge at 6000 x g for 1 minute. Discard the flow-through.
  2. Second Wash: Add Wash Buffer 2 to the column. Centrifuge at 8000 x g for 3 minutes to ensure complete removal of contaminants.

DNA Elution

  1. Elution: Place the column in a new collection tube. Add Elution Buffer directly to the center of the column membrane. Incubate at room temperature for 1 minute.
  2. Final Elution: Centrifuge at 6000 x g for 1 minute to elute purified genomic DNA.


Yield and Purity

The Maxi Whole Blood Genomic DNA Purification Kit AffiPURE typically yields 20-50 µg of genomic DNA per mL of whole blood, depending on the white blood cell count. The purity of the DNA is assessed by measuring the A260/A280 ratio, which generally falls between 1.8 and 2.0, indicating high-quality DNA suitable for downstream applications.


The integrity of the purified DNA is confirmed by agarose gel electrophoresis. High-molecular-weight DNA bands are observed, indicating minimal degradation during the extraction process.



The kit's protocol is straightforward and can be completed in approximately 60 minutes, making it suitable for routine use in research and clinical laboratories. The use of spin column technology ensures rapid processing and consistent results.


The purified DNA is compatible with various downstream applications, including PCR, qPCR, and NGS. The high purity and integrity of the DNA make it ideal for these sensitive techniques.

In conclusion, the Maxi Whole Blood Genomic DNA Purification Kit AffiPURE offers researchers a simple yet effective solution for isolating high-quality genomic DNA from whole blood samples. With its user-friendly protocol, high yield, and purity, this kit streamlines the DNA purification process, enabling researchers to focus more on their experiments and less on technical challenges. Whether in academic research labs or biotechnology companies, the AffiPURE kit proves to be a valuable tool in advancing genetic research and molecular diagnostics. 

Your Dynamic Snippet will be displayed here... This message is displayed because you did not provided both a filter and a template to use.

Streamlining Circulating DNA Purification: Evaluation of the Maxi Plasma Circulating DNA Purification Kit II AffiPURE