AffiPURE®​ Anti-DYKDDDDK-tag FAST IP kit

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CAT# AFG-LB-04
Size: 50 Tests

902.50 902.5 USD 902.50

902.50

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    AffiPURE®​ Anti-DYKDDDDK-tag FAST IP kit Background

    Anti-DYKDDDDK-tag fast IP kit is composed of Anti-DYKDDDDK affinity gel, Mouse IgG affinity gel, DYKDDDDK-Tag Rabbit pAb and Goat Anti-Rabbit IgG (peroxidase/HRP conjugated) , which is used for fast, efficient and specific (Co) IP of DYKDDDDK tag fusion protein. Anti-DYKDDDDK affinity gel is made by covalent coupling of high-quality DYKDDDDK-Tag Mouse mAb and Sepharose 4B agarose granules. It has the characteristics of high binding capacity of protein, high specificity and stability. Mouse IgG affinity gel is used as the control for CoIP experiment, and its properties are stable. The DYKDDDDK-Tag Rabbit pAb Antibody has the advantages of high specificity, high affinity and high valence; Goat Anti-Rabbit IgG (peroxidase/HRP conjugated) is purified by cross-adsorption and only DYKDDDDK-Tag Rabbit pAb is identified, and there is no cross-reaction with the heavy-chain and light-chain of the mouse monoclonal Antibody. The four components are strictly inspected and can be used independently; the combination of four sets has the advantages of fast, convenience and no interference band.


    Coupled Ligand

    Anti-Flag-Tag mouse monoclonal Antibody.

    Binding Capacity

    0.6 mg protein /mL agarose.

    Beads

    Sepharose 4B agarose with average particle size of 50 μm.

    Conjugate

    Agarose.

    Antibody Isotype

    IgG2a.

    Main Biomaterial Source

    Mouse.

    Product Category

    Affinity Gel/IP-CoIP.

    Recommended slurry Volume Per Run

    40 μL.

    Storage Buffer

    PBS with preservative and 50% glycerol, pH7.2.

    Storage Conditions

    (-20°C) for 12 months.